Invitrogen™ PureLink™ Plant RNA Reagent

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).

Brand:  Invitrogen™ 12322012

Product Code. 11528706

Includes: Supplied as a ready-to-use solution.

Description

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A₂₆₀/A₂₈₀ and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5g of tissue.

• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations
• Fewer resources used, less hazardous, less waste

Isolated RNA is ideal for a variety of applications and products:

• cDNA library construction kits
• Real-Time PCR (qPCR)
• Northern blotting kits and reagents
• Poly(A) selection kit RNase control reagents and assays

Not high-throughput compatible (Manual)

Specifications

• Content And Storage: PureLink™ Plant RNA Reagent is supplied as a ready-to-use solution. Store at +4°C.
• Purification Time: 1 hr.
• Final Product Type: Total RNA
• For Use With (Application): Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting
• Quantity: 1
• Starting Material Amount: Up to 1 g tissue, Up to 1 x 10^7 cells

• Isolation Technology: Organic Extraction
• Sample Type: Plant Samples
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 200 Preps
• Scale: Scaleable
• Target: Total RNA

Safety and Handling

Product Identifier

• PureLink  Plant RNA Reagent

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H311-Toxic in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H332-Harmful if inhaled.
• H373-May cause damage to organs.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P314-Get medical advice/attention if you feel unwell.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2-Mercaptoethanol, SODIUM AZIDE, lgepal

For Research Use Only. Not for use in diagnostic procedures.