Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, fluorescein

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Brand:  Thermo Scientific™ AB1220A

Product Code. 11842063

Description

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

• Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers
• The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets
• ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, low ROX concentration, fluorescein or no reference dye
• A master mix optimized for capillary instruments is also available

Highlights

• High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
• Store at -20°C

Specifications

• Content And Storage: Contains:
  • 1 x 5 mL ABsolute QPCR SYBR Green Fluorescein Mix (2X)
  •100 μL MgCl₂ (1M)
  Sufficient for 400 x 25 μL reactions.
  
  Store at -20°C
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
• Product Type: Real Time PCR SYBR Master Mix
• For Use With (Application): Gene Expression
• Concentration: 2X
• Sample Type: DNA, dsDNA

• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: ABsolute
• Sufficient For: 400 Reactions of 25 μL Each
• Compatibility: qPCR Cyclers
• No. of Reactions: 400 Reactions
• Volume: 5 mL

For Research Use Only. Not for use in diagnostic procedures.