3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%, Thermo Scientific Chemicals

Product Code	Brand	Quantity
15444629	Thermo Scientific Alfa Aesar J62216.06	5 g
15454629	Thermo Scientific Alfa Aesar J62216.09	10 g

Description

3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate

Application

• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques

• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3'-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces

• It is used as a chromogen for detecting the antigen-antibody complex

• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)

Chemical Identifiers

• CAS: 868272-85-9
• Molecular Weight (g/mol): 360.10
• InChI Key: KJDSORYAHBAGPP-UHFFFAOYSA-N
• PubChem CID: 23191111
• SMILES: Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1

• Molecular Formula: C12H18Cl4N4
• MDL Number: MFCD08273058
• Synonym: 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se
• IUPAC Name: 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate

Specifications

• CAS: 868272-85-9
• Molecular Formula: C12H18Cl4N4
• Synonym: 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se
• InChI Key: KJDSORYAHBAGPP-UHFFFAOYSA-N
• IUPAC Name: 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate
• PubChem CID: 23191111
• Physical Form: Powder

• Color: Gray
• MDL Number: MFCD08273058
• Solubility Information: Soluble at 5%(w/v) in water
• SMILES: Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1
• Molecular Weight (g/mol): 360.10
• Formula Weight: 360.11 (Anhydrous)
• Chemical Name or Material: 3,3'-Diaminobenzidine tetrahydrochloride hydrate

Safety and Handling

GHS H Statement
H351-H315-H319-H335
Suspected of causing cancer.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

P201-P202-P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P308+P313-P332+P313-P362-P501c

H315-H320-H335-H341-H350

missing translation for 'tsca' : Yes

Recommended Storage : Store at -20°C

RUO – Research Use Only