Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 15-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 15-well

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis. X2 Fermentas PageRuler(TM) Low Range Unstained

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). X2 PageRuler(TM) Unstained Protein (2 x 250 uL)

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 15 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions NuPAGE Novex 4-12% Bis-Tris Gel 1.0 mm, 15 well1 box/10 gels

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 10-well

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Prestained Protein

Invitrogen™ Novex™ BenchMark™ Protein Ladder, Unstained

Molecular weight standard for SDS-PAGE X2 Protein ladder BenchMark(tm) Store at -20C

Thermo Scientific™ Pierce™ Prestained Protein Molecular Weight Marker

A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE). 500 UL FERMENTAS PRESTAINED PROTEIN MOLECULARweight marker (SM0441), 6 bands (approx. 20,25,

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Plus Prestained Protei

Thermo Scientific™ Pierce™ 96-Well Microplates

Bind antibodies with high capacity using these specially formulated polystyrene plates for ELISA and other immunoassays. X25 Plate Pierce Reacti-Bind EIA white opaque

Invitrogen™ Novex™ NuPAGE™ 12% Bis-Tris Protein Gels, 1.0mm, 15 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bis-tris gel Invitrogen(tm) NuPAGE(r) Novex(r)

Agarose (Low-Melting, <1kb DNA/RNA/Genetic Analysis Grade), Fisher BioReagents

100GR Agarose (Low-Melting, less than 1kb DNA/RNA/Genetic Analysis Grade)

Hoefer™ SG Series Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride GRADIENT MAKER 50 ML

MetaPhor™ Agarose

500GR MetaPhor Agarose

Thermo Scientific™ Pierce™ Nylon Membranes

Sheets of unmodified or positively charged Biodyne A and Biodyne B Nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection. X25 Membrane Pierce nylon Biodyne A (pack of 25)

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00

Invitrogen™ Molecular Probes™ HPF (Hydroxyl Radical and Peroxynitrite Sensor)

Two indicators that together can selectively detect hypochlorite anion 3'-(P-HYDROXYPHENYL)FLUORESCEFsolution in DMF, 470 µl

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 10 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions NuPAGE Novex 4-12% Bis-Tris Gel 1.0 mm, 10 well1 box/10 gels

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 5 8Store in Fridge at 4C

GE Healthcare PlusOne™ Silver Staining Kit for Protein

Use for reproducible and sensitive staining of proteins in polyacrylamide gels. PlusOne™ Silver Staining Kit for Protein enables staining of proteins in less than two hours. SILVER STAINING KIT, PROTEIN

Thermo Scientific™ Pierce™ PageBlue™ Protein Staining Solution

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. PAGEBLUE PROTEIN STAINING SOLUTIONin Fridge at 4C

GE Healthcare Whatman™ Grade GB005 Blotting Paper

Recommended for applications where fewer layers of blotting paper must still ensure a high capacity. Whatman™ Grade GB005 Blotting Paper ensures the wicking capability and uniformity of capillary action that are important in obtaining clean and even transfers during blotting. X25 BLOTTING PAPER GB005 SHEETS CELLULOSE200mm x 200mmx 1.2mm (pack of 25)

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines eBioscience Foxp3 Staining Buffer Set FP=kit

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis. SUPERSIGNAL WEST FEMTO CHEMILUMINESCENT SUBSTRATETrial kit, SuperSignal 20mL Store in Fridge at 4C

Thermo Scientific™ Pierce™ Nickel Coated Plates

Bind His-tagged fusion proteins for ELISA and other plate-based assays to study interactions of expressed recombinant proteins. X5 Microplate strip Pierce HisGrab 8 well polystyr 5 Plates

Agarose (Low-EEO/Multi-Purpose/Molecular Biology Grade), Fisher BioReagents

CAS: 9012-36-6 Molecular Formula: C12H18O9 500GR Agarose, Low-EEO/Multi-Purpose, Molecular Biology Grade

Silica gel, C18-RP, 17%C, ca.0,8mmol/g, part. size: 40-63μ, ACROS Organics™

CAS: 71889-02-6 Molecular Formula: C18H37Cl3O2Si2 Molecular Weight (g/mol): 448.012 InChI Key: VYTRPUAMVCWPLO-UHFFFAOYSA-N Synonym: Octadecylsilicate PubChem CID: 172932 IUPAC Name: dioxosilane;trichloro(octadecyl)silane SMILES: CCCCCCCCCCCCCCCCCC[Si](Cl)(Cl)Cl.O=[Si]=O 250GR Silica gel, C18-RP, 17%C, approx. 0.7 mmol/g, part. size: 48-65 micron

Thermo Scientific™ Pierce™ HRP-Conjugated Streptavidin

Streptavidin from Streptomyces avidinii conjugated to highly purified horseradish peroxidase (HRP) HRP-CONJUGATED STREP 0,25ML

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5mm, 10 well

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bis-tris gel Invitrogen(tm) NuPAGE(r) Novex(r)

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