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Invitrogen™ ShhN (SHH) Human ELISA Kit

Sandwich ELISA Kit

£546.00 - £2320.00

Specifications

Accession Number Q15465
Assay Sensitivity 8 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate Biotin
Product Type ELISA Kit
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Product Code Brand Quantity Price Quantity & Availability  
15485123
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Invitrogen™
EHSHH
96 Tests
£546.00
1 set
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Estimated Shipment: 23-04-2024
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17155559
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Invitrogen™
EHSHHX5
5 x 96 Tests
£2320.00
Each
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Estimated Shipment: 30-04-2024
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Description

Description

The Human ShhN (SHH) ELISA quantitates Hu SHH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SHH. Principle of the method The Human SHH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Specifications

Specifications

Q15465
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
Human
Colorimetric Microplate Reader
HHG1,HLP3,HPE3,MCOPCB5,SMMCI,TPT,TPTPS
<10%
RUO
2°C to 8°C
Human
4 hr. 45 min.
8 pg/mL
Biotin
Proteases, Peptidases & Inhibitors
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
6469
<12%
HRP
Development
tcag7.582, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
1 hr. 20 min.
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