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Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1

For use in NucleoSpin™ Plasmid DNA purification kits for rapid small-scale preparation of high-copy plasmids from E. coli. Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1 without RNase A is a replacement component used in the NucleoSpin™ method's cell lysis process to resuspend the cell pellet.

Brand:  Macherey-Nagel™ 740911.1

Code : 86

Additional Details : Weight : 1.18000kg

Product Code. 12756553

  • £171.00 / 1000mL
Estimated Shipment: 19-04-2024
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Description

Description

The NucleoSpin™ Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mL E. coli overnight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.

After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing, highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used.

Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, all necessary buffers, RNase A, MN wash plate, MN tube strips, cap strips.

  • Silica-membrane technology
  • Yield: up to 20μL sequencing grade plasmid DNA
  • Elution volume: 75μL to 150μL
  • Culture volume: 1mL to 5mL
  • Binding capacity: 25μg
  • Maximum plasmid size <15kb
  • No use of organic solvents
  • NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included

For further information on this product contact your local Customer Services.

Specifications

Specifications

Resuspension Buffer
Room temperature (18-25°C)
NucleoSpin™ Plasmid DNA Purification Kits
Odorless
Resuspension A1 Buffer
No
Colorless
1g/cm3
For Plasmid DNA Purification
7.5–8.5
1000 mL
Solution
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