Cytiva Ready-to-Go™ You-Prime First-Strand Beads

Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. Cytiva Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.

Brand:  Cytiva 27-9264-01

Product Code. 10166214

Includes: Fifty preformulated, single-dose first-strand reaction mix beads prepackaged in white, thin-walled 0.5mL tubes compatible with most thermal cyclers. Each tube contains two beads containing buffer, dATP, dCTP, dGTP, dTTP, murine reverse transcriptase, RNase inhibitor, and RNase/DNase-free BSA. Also includes five control mix beads packaged in red tubes containing globin mRNA, buffer, and two globin-specific PCR primers for evaluating the performance of the Reaction Mix Beads and the PCR amplification.

Description

• Predispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance
• Each first-strand reaction requires only the addition of RNA in DEPC-treated water and a user-selected first-strand primer
• First-strand reaction may be primed with oligo(dT) primers, random primers such as pd(N)6, or custom primers complementary to a specific mRNA sequence
• Conditions of this reaction have been optimized to permit full-length transcription of RNAs 7kb or more in length
• Final reaction volume is 33μL once reconstituted
• Ideal for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples

Specifications

• Description: The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 mL tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
• Includes: Fifty preformulated, single-dose first-strand reaction mix beads prepackaged in white, thin-walled 0.5mL tubes compatible with most thermal cyclers, each tube contains two beads containing Buffer, dATP, dCTP, dGTP, dTTP, murine reverse transcriptase, RNase inhibitor and RNase/DNase-free BSA
• Product Type: Ready-To-Go You-Prime First-Strand Beads

• For Use With (Application): First-Strand
• No. of Reactions: 50