Thermo Scientific™ Pierce™ Protein-Free (TBS) Blocking Buffer

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Product Code	Brand	Quantity
10269613	Thermo Scientific™ 37570	1 L
13484299	Thermo Scientific™ 37585	100 mL

Description

Thermo Scientific™ Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Traditional blocking buffers contain proteins that can cross-react with immunodetection systems, resulting in high background and reduced signal. Typical commercial alternatives to protein-based blocking agents are ineffective. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes and other surfaces for ELISA, Western blotting, glass slide arrays and other applications.

Highlights:

• Protein-free blocker – minimizes or eliminates cross-reactivity associated with protein-based blocking buffers

• Application-compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides)

• Streptavidin-friendly – absolutely free of biotin; no possible interference with avidin-biotin detection systems

• High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

• Convenient – supplied as ready-to-use 1X formulations in TBS and PBS with and without 0.05% Tween™-20

Specifications

• Product Type: Protein-Free Blocking Buffer
• Product Line: Pierce™