Thermo Scientific™ Inclusion Body Solubilization Reagent

Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.

Brand:  Thermo Scientific™ 78115

Product Code. 11804091

Description

Effectively retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with Thermo Scientific™ Inclusion Body Solubilization Reagent.

The Inclusion Body Solubilization Reagent is a ready-to-use aqueous solution of denaturant that helps to dissolve aggregated proteins (inclusion bodies) that have been separated from the soluble fraction of bacterial cell lysates. The reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents such as Thermo Scientific B-PER Bacterial Protein Extraction Reagent. Bacteria are widely used for recombinant protein expression. However, 70%-80% of proteins produced by recombinant techniques in E. coli form inclusion bodies (i.e., protein aggregates). Once these aggregates are formed, it is very difficult to solubilize them. A proprietary denaturant contained in this reagent provides the most effective means for solubilizing aggregated proteins. Additional components, such as a reducing agent and a chelating agent, may be added to the reagent, depending on the particular application.

Highlights:

• Achieves the essential first step for subsequent insoluble protein refolding procedures
• One easy- and ready-to-use solution; no preparation needed
• Compatible with Thermo Scientific Coomassie Plus Protein Assay Kit (Part No. 23236) to determine degree of expression
• Proprietary denaturant can be easily removed by dialysis so that SDS-PAGE can be performed
• Can accommodate additional components such as reducing agents or chelating agents

Compatible with:

Thermo Scientific Pierce Protein Refolding Kit (Part No. 89867)

Specifications

• Product Type: Solubilization Reagent
• Form: Liquid
• Quantity: 100 mL

• Content And Storage: Upon receipt store at room temperature.
• Reagent Type: Chaotrope, Denaturant

Safety and Handling

Product Identifier

• Inclusion Body Solubilization Reagent

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : alkaline guanidine salt

For Research Use Only. Not for use in diagnostic procedures.