Macherey-Nagel™ NucleoSpin™ RNA Plant Kit

Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.

Product Code	Brand	No. of Reactions	Includes
12979894	Macherey-Nagel™ 740949.10	10 Preps	Lysis buffer RA1 (10mL), Lysis buffer RAP (10mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (6mL), Membrane desalting buffer (10mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size D vial), RNase-free water (13mL), NucleoSpin filters (10), NucleoSpin RNA Plan Columns with collection tubes (10), 2mL Collection tubes (30), 1.5mL Collection tubes (10)
12721021	Macherey-Nagel™ 740949.50	50 Preps	Lysis buffer RA1 (25mL), Lysis buffer RAP (25mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (12mL), Membrane desalting buffer (25mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size F vial), RNase-free water (13mL), NucleoSpin filters (50), NucleoSpin RNA Plan Columns with collection tubes (50), 2mL Collection tubes (150), 1.5mL Collection tubes (50)
12711021	Macherey-Nagel™ 740949.250	250 Preps	Lysis buffer RA1 (125mL), Lysis buffer RAP (125mL), Wash buffer RAW2 (80mL), Wash Buffer RA3 concentrate (3 x 25mL), Membrane desalting buffer (125mL), Reaction buffer for rDNase (30mL), RNase-free rDNase (5 size F vials), RNase-free water (60mL), NucleoSpin filters (250), NucleoSpin RNA Plan Columns with collection tubes (250), 2mL Collection tubes (750), 1.5mL Collection tubes (250)

Description

NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.

• Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
• rDNase included for on-column digestion—Efficient removal of contaminating DNA
• NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
• Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set

Kit Components:
NucleoSpin™ RNA columns with 2mL collection tubes, 2mL and 1.5mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water

• Mini spin column with silica membrane technology
• Yields 3–70μg RNA from 100μg plant material
• Elution volume: 40–60μL
• Fragment size: 200b–20kb
• Binding capacity: 200μg
• Preparation time: 30 min/6 preps
• A₂₆₀/A₂₈₀: 1.9–2.1

Specifications

• Format: Mini Spin Column
• For Use With (Application): RNA purification from plant cells and tissue
• Quantity: 1 Each
• Yield: 3–70μg

• For Use With (Equipment): Real-time RT-PCR
• Product Type: NucleoSpin™ RNA Plant Column
• Sample Size: <100mg tissue

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua