Macherey-Nagel™ NucleoSpin™ DNA RapidLyse

Economical kit for the rapid isolation and purification of genomic DNA from tail and ear clippings, organs, and cells in one hour or less. Kits with 10, 50 or 250 preps available.

Product Code	Brand	No. of Reactions
15795412	Macherey-Nagel™ 740100.10	10
15715422	Macherey-Nagel™ 740100.50	50
15705422	Macherey-Nagel™ 740100.250	250

Description

The isolation of genomic DNA (gDNA) can be a time-consuming task. The NucleoSpin™ DNA RapidLyse kit is designed with a unique buffer chemistry to minimize the expenditure of time, work, and money.

• Unique lysis chemistry to efficiently release gDNA from cells, tissues, and organs
• Powerful lysis in one hour or less
• Superior gDNA yields compared to standard extraction methods
• Highest cost-efficiency of gDNA purification products

Specifications

• Format: Mini Spin Column
• Sample Type: Eukaryotic Cells, Organs (Fresh, Frozen, Dried and Ethanol-Preserved), Tail and Ear Clippings
• Elution Volume: Elution volume: 60 - 100 μL
• For Use With (Application): Rapid extraction of genomic DNA
• Purity: A₂₆₀/A₂₈₀: 1.7 - 1.9
• Test Time: 60 min or less

• Isolation Technology: Silica Membrane Technology
• Efficiency: Binding capacity: 60 μg
• Final Product Type: genomic DNA
• Includes: Columns, 2mL Collection tubes, Buffers, Poteinase K
• Sample Volume: Up to 40 mg fresh weight, 1 x 10⁶ cells
• Yield: Up to 4 μg DNA per mg tissue

Safety and Handling

Product Identifier

• NucleoSpin DNA RapidLyse

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin sensitiser Category 1
• Serious eye damage/eye irritation Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H411-Toxic to aquatic life with long lasting effects.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.

Supplemental information

• MIXTURE LIST-Contains : proteinase K, liquid(origin: tritirachium album)/1-dodecylpyridiniumchloride,guanidinium thiocyanat