Macherey-Nagel™ NucleoSpin™ DNA Plasma Midi

Isolation of cell-free DNA from up to 5 mL blood plasma, utilizing the NucleoVac 96 Vacuum Manifold (sold separately, MPN 740681). The kit is designed for high recovery of circulating fragmented DNA ≥ 50 bp from human EDTA plasma.

Product Code	Brand	Product Type	Includes
15648455	Macherey-Nagel™ 740302.48	Core Kit	NucleoSpin Plasma Midi Columns, Collection Tubes, buffers, Proteinase K
15658455	Macherey-Nagel™ 740303.48	Full Kit	NucleoSpin Plasma Midi Columns, Collection Tubes, buffers, Proteinase K, Lysis Blocks

Description

The NucleoSpin™ DNA Plasma Midi kit is designed for the efficient isolation of circulating DNA from human blood plasma. Fragmented DNA of 50 bp and larger can be purified with high efficiency.

• Superior recovery of fragmented cell-free DNA
• Parallel purification of 24 samples—Adapter set for NucleoVac 96 Vacuum Manifold available
• Can be used with standard manual and automated vacuum manifolds
• Optimized protocol for Cell-Free DNA BCT™ (Streck Tubes)
• The kit is fully automatable on many liquid handling robots
• The protocol follows state-of-the-art bind—wash—elute procedures
• Ready to use for downstream applications like real-time PCR or NGS

Specifications

• Format: Midi Spin Column
• Sample Type: Blood Plasma
• Final Product Type: Cell-free DNA
• For Use With (Application): DNA isolation from blood plasma
• Purity: Fragment size: ≥ 50 bp
• Test Time: 90 min/24 preps

• Isolation Technology: Silica Membrane Technology
• Elution Volume: Elution volume: 200 μL (140 μL final eluate)
• For Use With (Equipment): Vacuum (e.g. NucleoVac 96 Vacuum Manifold and vacuum pump)
• No. of Reactions: 48
• Sample Volume: 1 - 5 mL
• Yield: Depends on sample, typically: 0.1 - 100 ng DNA/mL plasma