Macherey-Nagel™ NucleoBond™ RNA/DNA 400

Designed for the isolation and purification of up to 80μg or 400μg ultra pure total RNA

Brand:  Macherey-Nagel™ 740651

Product Code. 10181291

Description

NucleoBond™ RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80μg or 400μg ultra pure total RNA. A special manufacturing process guarantees RNase-free handling. Due to the superior resolving power of NucleoBond™ anion exchanger contaminations are washed away, and RNA and genomic DNA can be eluted separately.
Specific references:
M. Jiang et al. , J. Virol. Methods
38
(1992) 205T. Roitsch et al. , Plant Physiol.
108
(1995) 285D. Godt et al. , J. Plant Physiol.
146
(1995) 231R. Ehness et al. , The Plant Cell
9
(1997) 1825S. P. Hale et al. , Science
276
(1997) 1250Kit components: NucleoBond™ RNA/DNA 80 - NucleoBond™ AXR 80 columns, all required buffers. NucleoBond™ RNA/DNA 400 - NucleoBond™ AXR 400 columns, all required buffers.

• Anion-exchange technology
• Typical RNA yield: 70μg to 300μg from cultured cells 30μg to 150μg from tissue 50μg to 200μg from bacteria
• Sample material: eukaryotic cultured cells, tissue, bacteria/yeast cells
• Fragment size: 50bp to 300kb
• Binding capacity: 80μg for NucleoBond RNA/DNA 80 400μg for NucleoBond RNA/DNA 400
• Preparation time: 1.5hr to 2. 5hr
• Format: gravity-flow columns - midi or maxi

Specifications

• No. of Reactions: 10 Preps
• Product Type: RNA/DNA 400

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Skin corrosion/irritation Category 2

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : ethanol, guanidiniumthiocyanate, tritonx-100