Invitrogen™ Low Density Lipoprotein from Human Plasma (LDL)

Unlabeled LDL used to study normal cholesterol delivery and internalization

Brand:  Invitrogen™ L3486

Product Code. 10442132

Description

Human low-density lipoprotein (LDL) is large protein complex (approx. 500,000 Da) that binds to specific receptor on surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of the label can be analyzed after the specified time period.

• Label (Ex/Em): Unlabeled
• Acetylated: No
• Amount: 200 μL (2.5mg/mL)

Fresh LDL Produced Continually

• We prepare our LDL and AcLDL products from fresh human plasma approximately every two months
• Nonacetylated LDL products are shipped within two weeks of their preparation
• All acetylated LDL products are available on a continuous basis

Nonacetylated vs. Acetylated LDL

• LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization
• If the lysine residues of LDL«s apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess “scavenger” receptors specific for that modified form

Key Applications for Labeled LDL

• Applications for labeled LDL complexes include:
• Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization
• Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay)
• Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Wet Ice

Specifications

• Concentration: 2.5 mg⁄ml
• Detection Method: Fluorescence
• Dye Type: Unlabeled
• Shipping Condition: Wet Ice

• Content And Storage: Store in refrigerator (2–8°C).
• Product Type: Supplement
• Quantity: 200 μL

For Research Use Only. Not for use in diagnostic procedures.