Gibco™ Medium 199, Earle's Salts

Medium 199, Earle's Salts

Brand:  Gibco™ 31150022

Product Code. 11544426

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ Medium 199 product made in our Grand Island facility (11150-059). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Medium 199, 500 mL, 1X Concentration, Liquid Form, With L-glutamine, Phenol Red, 7.1 to 7.4 pH, 12 Months Shelf Life, 290 to 310 mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
• Concentration: 1X
• Product Type: Medium 199
• Without Additives: No HEPES
• pH: 7.1 to 7.4
• Regulatory Status: For in-vitro diagnostic use

• Content And Storage: Storage conditions: 2–8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin
• Form: Liquid
• With Additives: Glutamine, Phenol Red
• For Use With (Application): Mammalian cell culture
• Quantity: 500 mL