Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Red/Blue is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

Brand:  Invitrogen™ R37610

Product Code. 15450054

Includes: 3 x 2.5mL NucBlue Live, 3 x 2.5mL propidium iodide

Description

ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set.

• NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
• Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617nm)

Suggestions for use

• NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

Specifications

• Cell Type: Mammalian Cells, Eukaryotic Cells
• Content And Storage: Store at 2° to 8°C, or at room temperature.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Dye Type: 3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide
• Excitation Wavelength Range: 360, 535 nm
• Product Line: ReadyProbes
• Shipping Condition: Room Temperature

• Color: Blue, Red
• Description: ReadyProbes Cell Viability Imaging Kit, Blue/Red
• For Use With (Application): Viability Assay
• Product Type: Cell Viability Imaging Kit
• Emission: 460, 617 nm
• Format: Dropper bottles
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.