Invitrogen™ APRIL Human ELISA Kit

For the quantitative detection of human APRIL.

Brand:  Invitrogen™ BMS2008TEN

Product Code. 15591477

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human APRIL
• Biotin-Conjugate anti-human APRIL polyclonal antibody
• Streptavidin-HRP
• Human APRIL Standard lyophilized, 100ng/mL upon reconstitution
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Adhesive Films

Description

The Human APRIL (Hu A PRoliferation-Inducing Ligand)) ELISA quantitates Hu APRIL in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APRIL. Principle of the method The Human APRIL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. {ECO:0000269; PubMed:10973284}.

Specifications

• Accession Number: O75888
• Assay Sensitivity: 0.4 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: APRIL,CD256,TALL-2,TALL2,TNLG7B,TRDL-1,UNQ383/PRO715,ZTNF2
• Intraassay CV: 8.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 0.78-50.0 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 8741
• Interassay CV: 7.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: TNF 13, TALL 2, TRDL 1, CD256 antigen
• Test Time: 1 hr. 20 min.

For Research Use Only