Learn More
V5 Tag Monoclonal Antibody (SV5-Pk1), Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen R96025
188.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : 50
Additional Details : Weight : 0.00525kg
Description
NOTE: R960-25 was previously sold under product #46-0705. When re-ordering, please use #R960-25. R960CUS was previously sold under product #46-1157. When re-ordering, please use #R960CUS. This antibody recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. This antibody is functionally tested against 20 ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure. This antibody has also been tested in Western blot against 25 ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM). Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots. This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope.
V5 Tag Monoclonal antibody specifically detects V5 Tag in Tag samples. It is validated for ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot.Specifications
V5 Tag | |
Monoclonal | |
Unconjugated | |
simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag | |
V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. | |
50 μL | |
Primary | |
Antibody | |
IgG2a |
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot | |
SV5-Pk1 | |
PBS with 0.01% sodium azide | |
Mouse | |
purified | |
RUO | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid | |
Tag |