HIC Reagents and Supplies Hydrophobic Interaction Chromatography
Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ Sodium Deoxycholate Detergent
Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.
Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™
Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium Lauryl Sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads
Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.
Thermo Scientific™ Pierce™ Streptavidin Agarose
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL
Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.
Thermo Scientific™ PMSF Protease Inhibitor
Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Thermo Scientific™ Triton™ X-114 Surfact-Amps™ Detergent Solution
Purified nonionic Triton X-114 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
Thermo Scientific™ Brij™-35 Surfact-Amps™ Detergent Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA
Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 µg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 µg/mL.
Thermo Scientific™ HisPur™ Cobalt Spin Columns, 1mL
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Thermo Scientific™ Pierce™ Glutathione Agarose
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.