Centrifugal Filter Devices
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INCREASED VOLUME
FLEXIBILITY
QUICKLY CONCENTRATES BIOLOGICAL SAMPLES
PROVIDE CONCENTRATION AND DESALTING SOLUTIONS
UP TO 30-FOLD
CONCENTRATION
FOR FAST AND RELIABLE CLARIFICATION/FILTRATION
CONCENTRATE URIN AND CEREBROSPINAL FLUIDS
OFFERED WITH TWO MEMBRANE OPTIONS
FILTER VOLUMES AS LOW
AS 50 µL
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Complete your filtration process with these supplies:
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Eppendorf Centrifuge 5425/5425 R
Eppendorf’s newest 24-place microcentrifuges, Centrifuge 5425 and 5425 R, feature ergonomic operation and a large selection of rotors with a maximum speed of up to 21,300 × g, making them perfectly suited for all modern molecular biological applications in Eppendorf and PCR tubes from 0.2 to 5 mL.
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BRAND Centrifuge Tube Mini-Cooler
Designed to protect a wide range of solutions (enzymes, DNA, RNA, and cell suspensions) by helping to maintain freezer temperatures on the lab bench.
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Thermo Scientific™ F1-ClipTip™ Variable Volume Single Channel Pipettes
Lock tips firmly to the pipette with a complete seal with ClipTip technology, so they will not loosen or fall off. Lightweight handle design combined with minimal tip attachment and ejection forces.
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Vivaspin™ Tutorial: Choosing the Optimal Ultrafiltration Device
Key characteristics to consider when choosing the right centrifugal filter device.
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Frequently Asked Questions
How do I Choose the Correct MWCO Filter?
For proteins, it is recommended that the selected MWCO is three to six times smaller than the molecular weight of the solute being retained. If flow rate is a consideration, choose a membrane with an MWCO at the lower end of this range (3X). However, if the main concern is retention, choose a tighter membrane (6X). As different manufacturers use different molecules to define the MWCO of their membranes, it is important to perform pilot experiments to verify membrane performance in a particular application.
Can I Reuse MWCO Centrifugal Filters?
Although not ideal, MWCO filters can be reused if they don’t clog and provided that the proper cleaning protocol is followed. If there is drastic protein loss or concentration starts to take an unexpected long time, the filter is clogged. Despite this fact, they should be discarded after a maximum of three uses. These filters may start to leak after being used two to three times.
How do I Choose the Appropriate Centrifuge?
By using the proper rotor and adators to house your filtered tubes, it should be possible to reach the G force required in your filtration protocol.