Biological Buffers
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)
Use with Novex Tris-Glycine Gels
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled
Lonza™ AccuGENE™ PBS
Ready to use solutions ideal for a wide range of molecular biology applications
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311
Optimized for using with Novex™ pH 3-10 IEF gels
Invitrogen™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
Thermo Scientific™ Blocker™ BLOTTO in TBS
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ TE, pH 8.0, RNase-free
Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ IEF Cathode Buffer pH 3-10 (10X)
An optimized pre-mixed IEF cathod buffer
Invitrogen™ Novex™ Tris-Glycine Native Running Buffer (10X)
Use with NuPAGE Novex Tris-Acetate gels
Invitrogen™ Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675
Use with Novex Tricine Gels
Invitrogen™ Ammonium Acetate (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Cytiva Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.