Protein Extraction and Purification
Thermo Scientific™ RIPA Lysis and Extraction Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit
Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.
Cytiva (Formerly GE Healthcare Life Sciences) Streptavidin Sepharose™ High Performance Medium
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Cytiva (Formerly GE Healthcare Life Sciences) Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Cytiva (Formerly GE Healthcare Life Sciences) Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit
Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.
Invitrogen™ MagnaRack™ Magnetic Separation Rack
Holds up to 24 microcentrifuge tubes
Thermo Scientific™ Pierce™ IP Lysis Buffer
Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.
Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads
Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.
Cytiva (Formerly GE Healthcare Life Sciences) Mono Q PC, Columns Prepacked with MonoBeads™
Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. Cytiva Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.
Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Invitrogen™ 24 Deep-Well RB Block
Designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates
Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Cytiva (Formerly GE Healthcare Life Sciences) PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Thermo Scientific™ Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices.
Cytiva (Formerly GE Healthcare Life Sciences) HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns
Designed for method optimization and parameter screening
Thermo Scientific™ Pierce™ Spin Columns - Snap Cap
Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).
Invitrogen™ Exosome Spin Columns (MW 3000)
Fast, efficient removal of unincorporated dye from the labeled exosomes
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Triton™ X-100 (Electrophoresis), Fisher BioReagents™
CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
Thermo Scientific™ MagnaBind™ Carboxyl Derivatized Beads
Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.