Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.
Brand: Thermo Scientific™ 21277
Code : PN
Additional Details : Weight : 4.53590kg
DescriptionThe PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
6xHis pull-down – purifies protein interactors of any His-tagged fusion protein
Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples
Flexible – instructions include protocols for bait and prey proteins from different sources
Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes
Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates
|Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin
• Cobalt Chelate Agarose Resin, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Imidazole Solution (4M), 5 mL
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.