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Thermo Scientific™ Pierce™ PolyHis™ Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.

Brand:  Thermo Scientific™ 21277

Code : PN

Additional Details : Weight : 4.53590kg

Product Code. 10351214

  • £308.00 / Each


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Description

Description

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

  • 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein

  • Complete kit – provides all components and detailed protocol for purifying protein:protein interactions

  • No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)

  • Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples

  • Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates

Specifications

Specifications

Pierce PolyHis Protein Interaction Pull-Down Kit
Lysis Buffer, 250mL, Imidazole Solution (4M), 5mL, Microcentrifuge Collection Tubes, 100 tubes, TBS Pack (makes 500mL), 1 pack, Pierce Spin Columns and Accessories, 27 columns, Cobalt Chelate Agarose Resin, 750uL
At least 25 pull-down reactions each using 25uL of resin
Multi-component kit with cobalt agarose resin
25 rxn kit
Agarose
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