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Thermo Scientific™ Pierce™ IgG Elution Buffer, pH 2.0

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

Brand:  Thermo Scientific™ 21028

72.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : Weight : 1.00000kg


Product Code. 13454229

  • £108.00 / 1 L
Estimated Shipment: 11-04-2024
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Description

Description

Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G

  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

  • IgG Elution Buffer: pH 2.8; amine-based

  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers

Specifications

Specifications

Store at 4°C.
Elution Buffer
Elution Buffer
Pierce™
1 L
Liquid
Product Suggestions

Product Suggestions

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Safety and Handling

Safety and Handling

Product Identifier
  • Elution Buffer, pH 2

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information
  • MIXTURE LIST-Contains : Hydrogen chloride

SDS
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.