Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Amplify DNA directly from whole blood without prior purification

Brand:  Thermo Scientific™ F175S

Product Code. 15280114

Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction. This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions and from various species.

Highlights

• Direct PCR - sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available - allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire DNA polymerase - extremely short PCR protocol times
• Master Mix format with premixed gel loading dye - minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Includes

• 2X Phusion Blood Direct PCR Master Mix/li>
• 50 mM EDTA (pH 8.0)
• 50 mM MgCl₂ solution
• 100% DMSO
• Universal control primer mix
• Water, nuclease-free
• O'GeneRuler Express DNA Ladder

Specifications

• Content And Storage: Contains:
  • 1 mL Phusion Blood Direct PCR Master Mix (2X)
  • 40 μL Universal Control Primer Mix
  • 0.5 mL 50 mM EDTA
  • 1.5 mL 50 mM MgCl₂
  • 0.5 mL DMSO
  • 1.25 mL Water, nuclease-free
  • 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Line: Phusion
• Quantity: 100 x 20 μL Reactions
• No. of Reactions: 100 x 20 L Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• GC-Rich PCR Performance: High
• Polymerase: Phusion Blood II DNA Polymerase
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Blood

For Research Use Only. Not for use in diagnostic procedures.