Invitrogen™ EnzChek™ Direct Phospholipase C Assay Kit

Monitors PC-PLC activity

Brand:  Invitrogen™ E10215

Product Code. 10783734

Includes: 1 kit

Description

This kit provides continuous monitoring of phosphotidyl choline specific phospholipase C (PLC) activity in microplate based biochemical assays. The assay uses a PCL selective fluorogenic substrate that emits a bright green emission upon cleavage. PC-PLC plays a crucial role in many cell signaling pathways involved in apoptosis and cell survival, as well as diseases ranging from cancer to HIV1-7. The assay uses a glycerophospho-ethanolamine with a dye-labeled sn-2 acyl chain as a substrate for PC-PLC. Substrate cleavage by PC-PLC before the phosphate releases the dye-labeled diacylglycerol, which produces a positive fluorescence signal that may be measured continuously. The reaction product has absorption and fluorescence emission maxima of 509nm and 516nm, respectively. Using purified enzyme from Bacillus cereus, the assay can detect as little as 10mU/mL PC-PLC after one hour at room temperature. The kit has been proven useful for characterizing PC-PLC inhibition, and since it offers a direct measurement, the potential for false positives in a compound screen is eliminated.

• Each kit provides enough reagents for 10 microplates, using 200μL volumes in 96 well format

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Green
• Detection Method: Fluorescent
• For Use With (Equipment): Microplate Reader
• Dye Type: BODIPY™ FL
• Filter Type: FITC (Fluorescein) Filter
• Shipping Condition: Room Temperature
• Substrate Type: Phospholipase Substrate

• Content And Storage: I kit Store kit at -<20°, protected from light, desiccated
• For Use With (Application): Phospholipase Assay
• Product Type: Phospholipase Assay
• Emission: 509⁄516
• Product Line: EnzChek™, Molecular Probes™
• Substrate Properties: Lipid-Based Substrate, Chemical Substrate
• Target Enzyme: Phosphatidylcholine-Specific Phospholipase C

For Research Use Only. Not for use in diagnostic procedures.