BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 647, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen B35133

Product Code. 10335988

Description

Cell proliferation can be measured with BrdU following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody. Although this method is rapidly being replaced with the recently developed click chemistry-based Click-iTTM EdU assay, an advantage of the antibody clone MoBU-1 is that it does not cross-react with the thymidine analog EdU (5-ethynyl-2'-deoxyuridine), thus making it valuable for use in dual-pulse applications. Traditionally, the dual-pulse method, which is used for studying biological events such as neurogenesis and cell differentiation or evaluating anti-cancer drug efficacy, employs BrdU immunocytochemistry with iododeoxyuridine (IdU) or chlorodeoxyuridine (CldU), using multiple antibodies for detection. However, using sequential pulses of the thymidine analogs EdU and BrdU greatly simplifies dual-pulse labeling, and allows a straightforward and reliable method of distinguishing BrdU- and EdU-labeled cells. In addition to no cross-reactivity with EdU, the MoBU-1 clone has a high specificity for BrdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa FluorTM conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The approximate fluorescence excitation/emission maxima for Alexa FluorTM647 conjugate are 650/665 or 670 in nm.

Cell proliferation can be measured with BrdU following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody. Although this method is rapidly being replaced with the recently developed click chemistry-based Click-iTTM EdU assay, an advantage of the antibody clone MoBU-1 is that it does not cross-react with the thymidine analog EdU (5-ethynyl-2'-deoxyuridine), thus making it valuable for use in dual-pulse applications. Traditionally, the dual-pulse method, which is used for studying biological events such as neurogenesis and cell differentiation or evaluating anti-cancer drug efficacy, employs BrdU immunocytochemistry with iododeoxyuridine (IdU) or chlorodeoxyuridine (CldU), using multiple antibodies for detection. However, using sequential pulses of the thymidine analogs EdU and BrdU greatly simplifies dual-pulse labeling, and allows a straightforward and reliable method of distinguishing BrdU- and EdU-labeled cells. In addition to no cross-reactivity with EdU, the MoBU-1 clone has a high specificity for BrdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa FluorTM conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The approximate fluorescence excitation/emission maxima for Alexa FluorTM647 conjugate are 650/665 or 670 in nm. Detection with BrdU antibodies requires not only fixed and permeabilized cells, but also denatured DNA for the BrdU antibody to gain access to the incorporated BrdU. The appropriate dilution of the antibody and the incubation time should be determined empirically. We recommend the following starting points for Alexa FluorTM conjugate: 1-4 μg/mL (1:200-1:50 dilution). Labeled samples are compatible with all slide preparation methods, including wet mount and prepared mounting media (i.e., SlowFadeTM Antifade and ProLongTM Gold Kits).

Order Info

Shipping Condition: Wet ice

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5mM sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Content And Storage: 4° C
• Form: Liquid
• Target Molecule: Chemical

• Applications: Immunohistochemistry
• Clone: MoBU-1
• Conjugate: Alexa Fluor 647
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Immunogen: BrdU
• Quantity: 350 μL
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG

For Research Use Only. Not for use in diagnostic procedures.