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Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Synthesize long cDNAs up to 20 kb at elevated temperatures (up to 65°C) with this complete system for highly efficient synthesis of first strand cDNA.

Brand:  Thermo Scientific™ K1651

106.67 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 88

Additional Details : Weight : 0.01000kg

Product Code. 10338179

  • £160.00 / 20 reactions
Estimated Shipment: 16-04-2024
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Description

Description

Maxima H Minus First Strand cDNA Synthesis Kit† is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

The new Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Highlights

  • Integrated genomic DNA removal step
  • Increased reaction temperatures -the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
  • High yields of full-length first strand cDNA -with RNA templates up to 20 kb
  • Flexible priming -oligo(dT)18, random hexamer, or gene-specific primers
Specifications

Specifications

• Maxima H Minus Enzyme Mix
• Oligo(dT)18 and Random hexamer primers
• 5X RT Buffer
• dNTP Mix
• Nuclease-free water

Store at –20°C.

High
Reverse Transcription
Maxima H Minus
Dry Ice
First-Strand cDNA
Separate components
Up to 20 kb
Kit
30 min.
50°C to 55°C
Reduced
Real Time PCR (qPCR), RT-PCR
20 Reactions
Reverse Transcription
RNA
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This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.