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Invitrogen™ ShhN (SHH) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHSHH

Code : NEW

Additional Details : Weight : 0.33000kg

Product Code. 15485123

  • £414.00 / 1 set

Estimated Shipment date
from Supplier 23-12-2019

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Description

Description

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Specifications

Specifications

Q15465
Supernatant
40.96-10,000 pg/mL
Biotin
6469
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
100 μL per sample after dilution
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
96 assays
4 hr. 45 min.
Solid-phase sandwich ELISA
Development
tcag7.582, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
<10%
96 Tests
Human
8pg/mL to 10000pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Colorimetric Microplate Reader
ELISA Kit
8 pg/mL
Plasma, 50 μL|Serum, 50 μL|Supernatant, 100 μL
2°C to 8°C
1 hr. 20 min.
Human
Proteases, Peptidases & Inhibitors
SHH
<12%
HRP
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