Cytiva HiPrep™ IEX FF 16/10 Columns

Provide fast, reliable and reproducible preparative ion exchange separations

Product Code	Brand	Product Type	Ligand Type	Max. Pressure	pH Range
10607855	Cytiva 28-9365-41	HiPrep DEAE Sepharose FF column	Diethylaminoethyl	5 bar	2 to 12
10617855	Cytiva 28-9365-42	HiPrep CM Sepharose FF column	Carboxymethyl	1.5 bar	6 to 10

Description

DEAE and CM Sepharose™ 6 Fast Flow are prepacked in 20mL HiPrep columns. Ideal for rapid enrichments during initial capture of proteins from starting material.

Features:

• Combine good flowrates and high binding capacity
• Simple-to-operate
• Compatible with single-pump configurations and other chromatography systems (e.g., AKTA™)

Specifications (H₂O at room temp.)

• Dimensions: 1.6 x 10cm
• Recommended flowrate: 2-10mL/min. (60-300cm/h)
• Maximum flowrate: 10mL/min. (300cm/hr.)
• Max. pressure over packed bed: 1.5bar (22psi, 0.15MPa)
• Column hardware pressure limit: 5bar (73psi, 0.5MPa)
• Storage: 20% ethanol (DEAE,CM), 0.2M sodium acetate in 20% ethanol (SP)
• Storage temperature: 4° to 30°C

Specifications

• Buffer: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
• Diameter (English) Inner: 0.62 in.
• Quantity: 1 x 20mL
• Height (Metric): 100mm
• Material: Polypropylene
• Support Type: 6% Cross-linked Agarose
• Column Type: Ion Exchange Column

• Bed Volume: 20mL
• Diameter (Metric) Inner: 16mm
• Height (English): 3.93 in.
• Flow Rate: >300cm/hr.
• Particle Size: 45 to 165μm
• Content And Storage: 4° to 30°C
• For Use With (Application): Preparative separations of Proteins and other biomolecules using ion exchange (IEX) chromato graphy