Thermo Scientific™ Biotin Chromogenic Detection Kit

Perform chromogenic detection of biotinylated nucleic acid probes with the ThermoScientific™ Biotin Chromogenic Detection Kit.

Product Code	Brand	No. of Reactions
10755651	Thermo Scientific™ K0661	10
10899710	Thermo Scientific™ K0662	30

Description

Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit. 

Highlights

Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.

Highlights

• High sensitivity of detection - 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
• Low background due to optimized washing and blocking procedures
• Non-radioactive detection visualization does not require X-ray film or other specific equipment
• Fast and convenient - ready-to-use components