missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Biotin Chromogenic Detection Kit

Perform chromogenic detection of biotinylated nucleic acid probes with the ThermoScientific™ Biotin Chromogenic Detection Kit.

£105.00 - £253.00


Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10755651
View Documents
Thermo Scientific™
K0661
10
£105.00
10 reactions
View alternative products
Save Up to 
»
In stock, ready to ship: 2 Please to purchase this item. Need a web account? Register with us today!
10899710
View Documents
Thermo Scientific™
K0662
30
£253.00
30 reactions
View alternative products
Save Up to 
»
Estimated Shipment
01-02-2021
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit. 

Highlights

Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.

Highlights

  • High sensitivity of detection - 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
  • Low background due to optimized washing and blocking procedures
  • Non-radioactive detection visualization does not require X-ray film or other specific equipment
  • Fast and convenient - ready-to-use components
SDS