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Thermo Scientific™ ABsolute™ Probe-Based Detection qPCR Master Mixes

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

£114.00 - £1994.00

Specifications

Includes 16 x 1.25mL ABsolute QPCR Mix (2X)
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Products 4
Product Code Brand Description Quantity Price Quantity & Availability  
Product Code Brand Description Quantity Price Quantity & Availability  
11807682 View Documents Thermo Scientific™
AB1132B
ABsolute QPCR Mix, QPCR mix; 1.25mL vial; 800 rxns 16 x 1.25mL vials; 800 x 50µL rxns £1198.00
1600 reactions View alternative products
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11842073 View Documents Thermo Scientific™
AB1283B
ABsolute QPCR Capillary Mix, 2000 rxns 2000 x 20µL rxn £1190.00
2000 reactions View alternative products
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Estimated Shipment date
from Supplier 17-12-2019
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12687546 View Documents Thermo Scientific™
AB-1139/B
Absolute QPCR ROX (500nM) Mix, QPCR ROX mix; 5mL Vial; 2000 rxns 10 x 5mL vials; 2000 x 50µL rxns This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
N/A View alternative products
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11837682 View Documents Thermo Scientific™
AB-1138/A
Absolute QPCR ROX (500nM) Mix, QPCR ROX mix; 1.25mL vial; 100 rxns 2 x 1.25mL vials; 100 x 50µL rxns This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
N/A View alternative products
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Description

Description

ABsolute qPCR Master Mixes are designed to achieve the most consistent and reproducible data across all QPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and dTTP for increased reaction efficiency and sensitivity (low Ct).
  • Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification

  • Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time

  • Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates

  • Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

  • Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

  • Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination

Includes:

ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)

Recommended for:

Ideal for qPCR analysis in molecular biology applications, including gene expression and SNP typing

Specifications

Specifications

16 x 1.25mL ABsolute QPCR Mix (2X)

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.