Protein Extraction and Purification
GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Thermo Scientific™ Halt™ Protease Inhibitor Cocktails
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
GE Healthcare Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Invitrogen™ Molecular Probes™ EZQ™ Protein Quantitation Kit
Fluorescence-based protein assay facilitates fast quantitation of protein samples prepared for gel electrophoresis
Corning™ Spin-X™ UF Concentrators
Disposable, single use only ultrafiltration devices with polyethersulfone membranes (PES) for the centrifugal concentration and/or purification of biological fluids and aqueous solutions
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.
Thermo Scientific™ anti-HA Epitope Tag Clone: 2-2.2.14
HA Epitope Tag Monoclonal antibody 26181 from Thermo Scientific™ specifically detects HA Epitope Tag in Tag samples, and it is validated for Immunoprecipitation
Thermo Scientific™ Pierce™ Disposable Plastic Columns
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Thermo Scientific™ Pierce™ RIPA Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
GE Healthcare Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.
Invitrogen™ DYNAL™ DynaMag™ 50 Magnet
Optimized for efficient cell isolation using Dynabeads™
Thermo Scientific™ Pierce™ IP Lysis Buffer
Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads
Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Thermo Scientific™ NP-40 Detergent Surfact-Ams™ Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.
Merck Millipore BugBuster™ Protein Extraction Reagent
Reagent for protein sample preparation
GE Healthcare Streptavidin HP MultiTrap™ Plates
Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates
Sartorius™ Vivapure™ LentiSELECT 500 Purification and Concentration Kit
Fast purification of up to 3 x109 viral particles
GE Healthcare HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
GE Healthcare Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors